Ultrasonic Cell tawg Technology

Ultrasonic cell breakage yog hais txog technology ntawm kev rhuav tshem cell membrane thiab cell phab ntsa los ntawm sab nraud quab yuam tso tawm cell txheem nrog rau lub hom phiaj cov khoom xyaw. Nws yog lub hauv paus ntawm kev sib cais thiab purification ntawm cov khoom uas tsis yog-secretory biochemical (cov khoom) synthesized hauv hlwb. Ua ke nrog kev nce qib loj hauv kev siv thev naus laus zis DNA thiab cov ntaub so ntswg kab lis kev cai, cov proteins yav dhau los xav tias nyuaj kom tau txais tam sim no tuaj yeem tsim tawm ntau. Tab sis vim yog cov phab ntsa ntawm tes ntawm cov kab mob, cov poov xab, cov kab mob thiab cov nroj tsuag yog tsim los ntawm cov khoom sib txawv, thiab tib hom cell tsim cov tes hauj lwm sib txawv, lawv cov phab ntsa ntawm tes muaj ntau theem ntawm kev ruaj khov. Tsiaj cells tsis muaj cov phab ntsa ntawm tes, tab sis muaj cov cell membranes, uas tseem xav tau qee txoj kev rhuav tshem ntawm tes kom ua tiav lub hom phiaj ntawm cov khoom rho tawm. Ultrasonic cell breakage feem ntau muaj thermal nyhuv, cavitation nyhuv thiab mechanical nyhuv ntawm cov hlwb. Cov nyhuv thermal yog tias thaum ultrasound yog propagated nyob rau hauv nruab nrab, kev sib txhuam impedes lub molecular kev co tshwm sim los ntawm ultrasound, ua rau ib feem ntawm lub zog yuav tsum tau hloov dua siab tshiab rau hauv lub zos kub kub. Cavitation nyhuv yog tsim ntawm cavitation nyob rau hauv lub cev nyob rau hauv ultrasonic irradiation. Nrog rau kev vibration ntawm cavitation thiab nws nruj implosion, mechanical shear siab thiab turbulence yog generated, uas ua rau qog los ntshav, cov ntaub so ntswg vau thiab necrosis. Cov nyhuv mechanical yog thawj cov nyhuv ntawm ultrasound. Thaum lub sij hawm ultrasonic propagation, qhov nruab nrab hais alternately compress thiab stretch, uas ua rau cov kev hloov ntawm siab thiab kev puas tsuaj ntawm cell qauv. Cov nyhuv tua yog ze ze rau qhov zaus thiab kev siv ntawm ultrasound. Lub hauv paus ntsiab lus ua haujlwm ntawm ultrasonic cell crusher yog ua raws li cov nyhuv cavitation ntawm ultrasonic nthwv dej hauv cov kua. Lub transducer tsim lub zog siab shear quab yuam nyob rau hauv cov kua nyob rau sab saum toj ntawm lub cuab yeej lub taub hau los ntawm lub zog hluav taws xob ntawm lub horn, tsim cov zaus hloov dej siab, uas ua rau cov kab noj hniav nthuav dav, tawg thiab tsoo lub hlwb. Ntawm qhov tod tes, vim muaj kev kub ntxhov tshwm sim los ntawm ultrasonic nthwv dej propagation nyob rau hauv cov kua, cov hais ua ib tug zoo acceleration, thiaj li mus sib tsoo nrog ib leeg los yog nrog cov phab ntsa thiab smash. Siv dav hauv biochemistry, tshuaj kho mob, kev tshawb nrhiav zaub mov, kev saib xyuas ib puag ncig thiab lwm thaj chaw ntawm cov tsiaj txhu thiab cov nroj tsuag tawg, cov kab mob, cov kab mob thiab lwm cov qauv ntawm tes nrog rau hauv emulsification, sib cais, homogenization thiab lwm yam tshuaj tiv thaiv txheej txheem.